GENETIC IDENTFICTATION OF INDIVIDUAL HOST
BLOOD FROM AFRICAN TSESTE FLIES
This on-going project was initiated in 1997 at Trent University with
Dr S. Schoffield and Dr S. Torr of the Natural Resources Institute
(NRI), Greenwich, UK.
The Wildlife Forensic DNA Laboratory extracts DNA from tstetse fly
blood meals that have been collected in experimental fenced and netted
enclosures in Tanzania. Individual-specific DNA profiles
are generated using bovine-specific microsatellite loci that provide
the identification of single or multiple blood meals by a tsetse and
specifically which cattle have been fed upon.
The objective of this study is to determine the feeding preference
of African tsetse. In addition to the academic interest in biting-fly
feeding behaviour, the applied management implications of this study
are that by identifying tsetse preferred cattle recommendations can
be made to local farmers as to which cattle should be immunised against
Trypsanimosis, sleepling sickness. 1,000,000 people and cattle
are infected by Tsetse flies every year in Africa.
Approximately 500 blood meals were analysed in 1999-2000 and approximately
750 were profiled in 2000-2001.
Natural Resources DNA Profiling
and Forensic Centre
DNA Building, Trent University,
2140 East Bank Drive, Peterborough, Ontario, Canada, K9J 7B8
Phone: (705) 748-1133 | Fax (705) 748-1132