Blood Sample Collection

Blood Sample Submission

Preparation of Blood for DNA Analysis

DNA in blood samples can undergo rapid degradation in less than a week unless preserved in some manner such as blood frozen on snow. Blood stains on rocks, leaves or clothing may be problematic due to contamination by microorganisms or in the case of clothing dyes.

Blood stains are found on two sources:
– Non-absorbent material e.g. Metal and nylon
– Absorbent material e.g. Cloth and soil

Dry Blood Samples
1. Using new gloves remove the swab from the tube.
2. Dip the swab into the Phosphate Buffered Saline (PBS) which is supplied with the kit (Do not dip the swab into the PBS a second time!)
3. Wipe the blood stained area gently with the swab.
4. Allow the swab to air dry and replace it into the tube from which it came.
5. Place the tube into a Ziploc bag with a desiccant pouch to absorb any additional moisture.

NOTE: If the area is difficult to swab or the amount is too little place the blood stained object into a Ziploc bag with a desiccant pouch.

Wet Blood Samples
1. Using new gloves remove the swab from the tube.
2. Wipe the area, which contains the blood gently with the swab.
3. Allow the swab to air dry and replace it into the tube from which it came.
4. Place the tube into a Ziploc bag with a desiccant pouch to absorb any additional moisture.

NOTE: If the amount of blood is very little or it is on organic material place the object into a Ziploc bag with a desiccant pouch or into one of the desiccant tubes, which have desiccant beads in the bottom.

Whole Blood Samples from Live Animals
1. Using new gloves puncture the animal with a needle; enough to allow for a few drops of blood or you can use a needle and syringe to remove a small amount of blood.
2. Remove the insert from the Blood Collection Envelopes
3. On the inside of the insert there is two circles on an absorbent paper, this is where you want to put the drops of blood.
4. Repeat step 3 with the second circle of the absorbent paper.
5. Allow the blood to air dry and replace the insert into the envelope from which it came.
6. Do not seal the envelope.
7. Place the envelope into a Ziploc bag with a desiccant pouch to absorb any additional moisture.

Comments are closed.